Fig. 2. Continuous evolution of synthetic auxotrophs leads to adaptation to lower BipA concentration rather than escape.
(A) Timeline for continuous evolution, with detection limits for escape frequency assays shown in parentheses. (B) Doubling times of progenitor and evolved synthetic auxotrophs as a function of BipA concentration, normalized to the doubling time of DEP at 100 μM BipA. Error bars represent the SD across technical triplicates within the same experiment.
