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. 2021 Jul 2;7(27):eabf5851. doi: 10.1126/sciadv.abf5851

Fig. 2. Continuous evolution of synthetic auxotrophs leads to adaptation to lower BipA concentration rather than escape.

Fig. 2

(A) Timeline for continuous evolution, with detection limits for escape frequency assays shown in parentheses. (B) Doubling times of progenitor and evolved synthetic auxotrophs as a function of BipA concentration, normalized to the doubling time of DEP at 100 μM BipA. Error bars represent the SD across technical triplicates within the same experiment.