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. 2021 Jul 2;7(27):eabg0088. doi: 10.1126/sciadv.abg0088

Fig. 2. Acute response to dexamethasone in WT and Actn3 KO mice.

Fig. 2

(A) Actn3 KO mice show normal transcriptional response to GR activation at 3 and 24 hours after a single bolus injection of dexamethasone but fail to activate downstream E3 ubiquitin ligase genes (B) Fbxo32 or (C) maintain Trim63 up-regulation at 24 hours after dexamethasone treatment. a.u., arbitrary units. (D) Relative to WT-Sal controls, WT-Dex mice show significant down-regulation of p-S6RP, total S6RP, and p-4ebp1 and no changes to the total 4ebp1 at 24 hours after dexamethasone treatment, consistent with the dexamethasone-induced suppression of protein synthesis signaling. (E) In contrast, Actn3 KO-Dex mice are resistant to these changes and show similar levels relative to KO-Sal controls over the same time period. All transcriptional and protein analyses were performed in the quadriceps muscle. *P < 0.05 and **P < 0.01, Mann Whitney U test. N = 4 to 7 for all experiments.