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. 2021 Apr 2;7(14):eabd1866. doi: 10.1126/sciadv.abd1866

Fig. 4. IL-7Rα blockade reversed early-onset AA in C3H/HeJ mice.

Fig. 4

Four to 5 weeks after AA skin grafting, C3H/HeJ skin-grafted mice with early-onset AA were administered control mAb (n = 10) or anti–IL-7Rα mAb (n = 10) by intraperitoneal injection two times weekly for 8 weeks. (A) Representative images of anti–IL-7Rα or control Ab–treated C3H/HeJ mice before and after treatment. (B) Survival curve analysis demonstrates the hair regrowth between the two groups. **P < 0.01 (log-rank test). (C) Representative immunofluorescence images of skin sections from the two groups, stained with anti-CD8, anti–MHC-I, or anti–MHC-II Abs. Scale bars, 100 μm. (D) Single-cell suspensions of skin cells were gated on CD45 and CD3 for flow cytometry analysis, or the cells were stimulated with phorbol 12-myristate 13-acetate/ionomycin for cytokines expression by intracellular flow cytometry. Top: Representative flow cytometric plots data showing immune cell profiles in skin of C3H/HeJ mice from the two groups. Bottom: Summary graphs of various percentages of immune cells. ***P < 0.001 (unpaired Student’s t test). (E and F) RNA-seq analysis from treated mice presented as a heatmap (E) and as a cumulative ALADIN index (F). Photo credit: Mice pictures taken by Zhenpeng Dai, Columbia University.