Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 2;7(27):eabf1973. doi: 10.1126/sciadv.abf1973

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

PMC Copyright notice

Fig. 3 — (A to C) Zeb2 represses neuronal adhesion. (A) Surface proteins on Zeb2^fl/fl Nex^Cre and Zeb2^fl/fl cortical neurons prepared from E15.5 embryos were identified by biotin-linked mass spectrometry. DIV, days in vitro. (B) Top Gene Ontology (GO) terms for surface proteins up-regulated ≥1.5× upon loss of Zeb2. ECM, extracellular matrix; KEGG, Kyoto Encyclopedia of Genes and Genomes. (C) GO of surface-expressed proteins. Red nodes are up-regulated in Zeb2^fl/fl Nex^Cre and blue nodes are up-regulated in Zeb2^fl/fl animals. ATPase, adenosine triphosphatase; MAPK, mitogen-activated protein kinase; RTK, receptor tyrosine kinase. (D and E) Zeb2 suppresses neuronal aggregation. (D) Aggregation of single-cell suspensions over time. Scale bars, 100 μm. (E) Average cell aggregate size. N = 15, 10, and 7 Zeb2^fl/fl and 12, 12, and 15 Zeb2^fl/fl Nex^Cre aggregates at 0, 30, and 60 min, respectively. One-way ANOVA with Kruskal-Wallis test. (F and G) Zeb2 inhibits adhesion to the extracellular matrix. (F) Attachment of neuronal suspensions to laminin-coated surfaces after 2 hours. Scale bars, 15 μm. (G) Lamellipodial spreading of attached cells. N = 42 Zeb2^fl/fl and 56 Zeb2^fl/fl Nex^Cre cells. Mann-Whitney test. (H to J) Zeb2 regulates radial migration through suppression of integrin signaling. (H) IUE of GFP and ItgB1DN into Zeb2^fl/fl and Zeb2^fl/fl Nex^Cre E14.5 littermate animals. (I) GFP⁺ neurons at E18.5. Scale bar, 100 μm. (J) Laminar distribution of GFP⁺ neurons. N = 12 Zeb2^fl/fl, 5 Zeb2^fl/fl Nex^Cre, and 5 Zeb2^fl/fl Nex^Cre + ItgB1DN animals. Two-way ANOVA with Bonferroni post hoc test.