Fig. 6. Zeb2 regulates the orientation and dendritic arborization of UL neurons.

(A) Zeb2^fl/fl and littermate Zeb2^fl/fl Nex^Cre animals were in utero electroporated at E15.5 with GFP. (B) Representative images of GFP⁺ UL neurons showing the orientation of the dendritic tree with respect to the pia (marked by a dashed line) at P23. Arrowheads mark the axon. Scale bars, 50 μm. (C) Tracings of P23 GFP⁺ WT and KO neurons. The dashed line indicates the position of the pia. Apical dendrites are shaded green. Arrows indicate orientation of the apical dendrite. Scale bar, 50 μm. (D) Proportion of cells with aberrant orientation at P23. N = 65 cells per condition. (E) Average length of the apical dendrite at P23. N = 65 cells per condition. Welch’s t test. (F to H) Sholl analysis of the complexity of the dendritic arbor of WT (Zeb2^fl/fl) and KO (Zeb2^fl/fl Nex^Cre) neurons at P23. (F) Sholl curve showing the number of dendritic intersections at increasing distance from the soma. N = 30 neurons, 10 neurons from three different animals per condition. Unpaired t test with Welch’s correction. (G) Average number of dendritic intersections per cell. Mann-Whitney test. (H) Average number of primary dendrites per cell. Unpaired t test with Welch’s correction.