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. 2021 Jul 2;7(27):eabf1973. doi: 10.1126/sciadv.abf1973

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Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 7 — (A and B) Zeb2 regulates neuronal orientation from P2. (A) IUE of Zeb2^fl/fl and Zeb2^fl/fl Nex^Cre littermate animals with GFP at E15.5. (B) GFP⁺ neurons were analyzed at different time points. Arrowheads indicate aberrantly oriented neurons. Scale bars, 50 μm. (C) Deviation of the apical dendrite. N = 40, 36, 33, and 65 Zeb2^fl/fl and 33, 56, 22, and 65 Zeb2^fl/fl Nex^Cre neurons at E18.5, P2, P7, and P23, respectively. Mann-Whitney test. (D to I) Nrp1 regulates dendritic complexity but not neuronal orientation. (D) IUE of Zeb2^fl/fl animals at E15.5 with shScr, Cre, or shNrp1 as indicated. (E) Traces of GFP⁺ neurons at P23. Apical dendrites are shaded green. Arrows indicate orientation of the apical dendrite. Scale bars, 50 μm. (F and G) Sholl analysis at P23. N = 30 Zeb2^fl/fl + shScr cells (from five animals), 39 Zeb2^fl/fl + Cre + shScr (six animals), and 30 Zeb2^fl/fl + Cre + shNrp1 (seven animals). (F) Average number of dendritic intersections per cell. One-way ANOVA with Bonferroni’s multiple comparison test. (G) Dendritic complexity at P23. Unpaired Student’s t test. (H) Representative GFP⁺ neurons at P23. (I) Deviation of the apical dendrite. N = 143 Zeb2^fl/fl + shScr (five animals), 49 Zeb2^fl/fl + Cre + shScr (six animals), and 64 Zeb2^fl/fl + Cre + shNrp1 neurons (seven animals). One-way ANOVA (Kruskal-Wallis) with Dunn’s multiple comparison tests.