Fig. 9. Cdh6 regulates apical dendrite orientation downstream of Zeb2.

(A) Zeb2^fl/fl animals were in utero electroporated at E15.5 with GFP, Cre, shScr, or shCdh6 as indicated. (B) Representative images of GFP⁺ neurons in brain slices at P23. The apical dendrite is marked by a fine parallel tracing; the pia is marked by a thicker dashed line. Scale bar, 50 μm. (C) Tracings of 10 apical dendrites per condition showing their orientation with respect to the pia. Apical dendrites were superimposed so that all deviations face the right-hand side of the image. Scale bar, 50 μm. (D) Average deviation of the apical dendrite. N = 143 Zeb2^fl/fl + shScr cells (seven animals), 57 Zeb2^fl/fl Nex^Cre + Cre cells (five animals), and 85 Zeb2^fl/fl Nex^Cre + shCdh6 cells (four animals). One-way ANOVA (Kruskal-Wallis test) with Dunn’s multiple comparison test.