FIG. 5.

VLDLR fragments protect HeLa cells against infection with HRV2. HRV2 (100 TCID₅₀) was incubated for 90 min at 34°C with serial twofold dilutions (left to right) of HeLa cell supernatant obtained upon incubation with PBS (S), of fraction 2 (F2) and of fraction 9 (F9) as eluted from the GST-RAP column (see Fig. 4), and of corresponding F2 and F9 of the GST-RAP column purification of rVLDLR_1–8h. Cells infected with virus preincubated with plain infection medium (MIM) were used as the negative control. HeLa cell monolayers were challenged with the mixtures and stained with amido black after incubation for 3 days at 34°C. MIM and supernatant from the PBS incubation (S) were also compared in the absence of virus to exclude any influence on HeLa cell viability.