FIG. 2.

Stimulation of the ATPase activities of WT and mutant T antigens by (oligo)dT. WT (A) and 259-708 (B) T antigens were purified by immunoaffinity with PAb101 as described elsewhere (35) and assayed for ATPase activity in the presence of different amounts of oligo(dT). The free inorganic phosphate (Pi) was separated from the ATP substrate by ascending thin-layer chromatography. The origin is shown on the right. Lanes 1, no T antigen; 2 through 7, 0, 2, 4, 8, 20, and 40 ng of oligo(dT), respectively. (C) The amounts of released inorganic phosphate were quantitated by scintillation counting and plotted against the amount of oligo(dT) in each reaction.