Figure 2.

Cell viability assays after OV infection

(A) Patient-derived cell cultures were incubated with various dilutions multiplicities of infection (M.O.I.) of Delta24-RGD, rQNestin34.5v1, R124, and rNDV-F0-GFP and cell viability was measured 5 days post OV infection with CTG assay. The data are shown as the percentage of cells alive after infection with Delta24-RGD, rQNestin34.5v1, R124, or rNDV-F0-GFP at the indicated log10 (M.O.I.s) relative to the non-infected cells, with each point corresponding to the mean ± (SD) values of independent CTG assays per cell line (n = 2 to 4) (see also Table S1). (B) EC₅₀ obtained from (A), the color scaling is independent per OV from green (lowest EC₅₀) to red (highest EC₅₀). (C) Mean EC₅₀ comparisons grouped per tumor entity. Each point in the bar plots corresponds to the EC₅₀ of a specific PBT cell culture. For the comparison of the EC50 per tumor entity the non-parametric Kruskal-Wallis test with Dunn’s multiple comparison correction. ns = not statistically significant.