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. 2024 Apr 30;15:3610. doi: 10.1038/s41467-024-47300-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Number of nNOS neurons in the VMHvl. One-way ANOVA; F(2,9) = 22.47, p = 0.00031. **p = 0.0086; ***p = 0.0003. b the number of nNOS expressing GR. One-way ANOVA: F(2,9) = 2.519, p = 0.1353. ns = not significant. c Percentage of nNOS expressing GR in the VMHvl. One-way ANOVA: F(2,9) = 1.06, p = 0.385. Control (n = 3), highly receptive (HR) females (n = 4), Minimally receptive (MR) females (n = 5). d Representative confocal images showing nNOS/GR immunostaining in the VMHvl in a control subject vs a MR female. White dashed box indicates the region of high magnification. Full arrow shows an example of colocalization between nNOS and GR, whereas hollow arrow points to nNOS expression only. e Number of nNOS neurons in the PVN. One-way ANOVA: F(2,9) = 1.789, p = 0.221. f Number and (g) percentage of nNOS neurons in the PVN expressing GR. One-way ANOVA; number of nNOS neurons expressing GR (F(2,9) = 0.203, p = 0.819), Percentage of nNOS expressing GR (F(2,9) = 0.826, p = 0.468). Control (n = 5), HR females (n = 4), MR females (n = 3). h Representative confocal images showing nNOS/GR immunostaining in the PVN in a control vs a MR female. White dashed box indicates the region of high magnification. Full arrow shows an example of colocalization between nNOS and GR, whereas hollow arrow points to nNOS expression only. All data are presented as mean ± SEM. Corrections were made whenever multiple comparisons test is used. Source data are provided as a Source Data file.