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. 2024 Apr 30;15:3610. doi: 10.1038/s41467-024-47300-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Representative confocal images showing nNOS and PR coexpression in the VMHvl in control and minimally receptive (MR) females. White dashed box shows the region of high magnification. Red arrow shows an example of a nNOS neuron expressing PR. White arrow indicates an example of a PR neuron that is not coexpressing nNOS, while white hollow arrow points to nNOS expression only. b Venn diagram representing the two populations of nNOS and PR neurons in the VMHvl. c Number of nNOS neurons in the VMHvl. One-way ANOVA; F(2,10) = 131.91, p = 0.0013. *p = 0.0331, **p = 0.011; Bonferroni’s multiple comparisons test. d Number of PR neurons in the VMHvl. One-way ANOVA: F(2,10) = 0.06, p = 0.941. e number of nNOS neurons colocalizing with PR. One-way ANOVA: F(2,10) = 4.329, p = 0.0442. *p = 0.0491; Bonferroni’s multiple comparisons test. n = 5 for control, n = 4 for HR females, and n = 4 for MR females. f Representative confocal images showing nNOS and ERα coexpression in the VMHvl in control and MR females. White dashed box shows the region of high magnification. Solid white arrow shows an example of ERα expressing neuron coexpressing nNOS. White hollow arrow indicates an example of a nNOS neuron that is coexpressing ERα. g Venn diagram representing the distribution of nNOS and ERα and the percentage of their coexpression in the VMHvl. h Number of nNOS neurons in the VMHvl. One-way ANOVA followed by Bonferroni’s post hoc test: F(2,10) = 11.91, p = 0.0023. *p = 0.0331, **p = 0.0028; Bonferroni’s multiple comparisons test. i Number of ERα neurons in the VMHvl; F(2,11) = 1.698, p = 0.227. j Number of nNOS neurons colocalizing with ERα in the VMHvl. F(2,10) = 10.52, p = 0.0035. **p = 0.004; Bonferroni’s post hoc test. Control (n = 4), HR females (n = 3), MR females (n = 6). All data are presented as mean ± SEM. Adjustments were made whenever multiple comparisons test is used. Source data are provided as a Source Data file.