Table 3.
Antibodies Used for Western Blot and Immunofluorescence
| Antibodies | Catalog no. | Vendor | Usage | RRID |
|---|---|---|---|---|
| Armenian hamster anti-CD31 | ab119341 | Abcam | 1:200 IF | AB_10900179 |
| Anti-Lyve-1 antibody | ab218535 | Abcam | 1:200 IF | AB_2927473 |
| Goat anti-mouse Lyve-1 | AF2125 | R&D | 1:50 IF | AB_2297188 |
| Antilaminin antibody | ab11575 | Abcam | 1:200 IF | AB_298179 |
| Anti-VEGF receptor 2 | 2479 | Cell Signaling Technology | 1:1000 for WB; 1:100 for IP | AB_2212507 |
| Anti-VEGF receptor 2 (phospho-Y1054 + Y1059) | ab5473 | Abcam | 1:250 IF | AB_304917 |
| Anti–phospho-VEGF receptor 2 (Tyr1175) | 2478 | Cell Signaling Technology | 1:1000 WB | AB_331377 |
| Anti–β-tubulin antibody | sab4200715 | Sigma | 1:2000 WB | AB_2827403 |
| Anti–cytochrome P450 2E1 antibody | ab28146 | Abcam | 1:500 IF | AB_2089985 |
| Anti-CD36 antibody | ab252923 | Abcam | 1:1000 WB | NA |
| Cy3 AffiniPure goat anti-Armenian hamster IgG (H+L) | 127-165-099 | Jackson Lab | 1:400 IF | AB_2338988 |
| Goat anti-rat IgG (H+L) Alexa Fluor 647) | A-21247 | Invitrogen | 1:500 IF | AB_141778 |
| Donkey anti-goat IgG H&L (Alexa Fluor 488) | ab150133 | Abcam | 1:200 IF | AB_2832252 |
| Donkey anti-rat IgG (H+L) (Alexa Fluor 647) | A-21244 | Invitrogen | 1:200 IF | AB_2535812 |
| Goat anti-rabbit IgG H&L (Alexa Fluor 488) | ab150077 | Abcam | 1:200 IF | AB_2630356 |
| RCA I-fluorescein | FL1081 | Vector Lab | 1:500 IF | AB_2336708 |
| Biotinylated maackia amurensis lectin II | B-1265 | Vector Lab | 1:200 IF | AB_2336569 |
| Biotinylated sambucus nigra lectin | B-1305-2 | Vector Lab | 1:200 IF | AB_2336718 |
| Horseradish peroxidase–conjugated streptavidin | 405210 | BioLegend | 1:2000 IF | NA |
| Anti–glutamine synthetase antibody | ab176562 | Abcam | 1:200 IF | AB_2868472 |
| Anti-mouse E-cadherin (clone DEMCA-1) (Alexa Fluor 594) | 147306 | BioLegend | 1:200 IF | AB_2563230 |
| Anti-CD34 antibody | ab81289 | Abcam | 1:50 IF | AB_1640331 |
DEMCA-1, clone number; H&L, heavy and light chains of immunoglobulin G; IF, immunofluorescence; IP, immunoprecipitation. NA, not available; RCAⅠ, Ricinus communis agglutinin Ⅰ; RRID, Research Resource Identifier; WB, Western blot.