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. 2024 Apr 26;103:105132. doi: 10.1016/j.ebiom.2024.105132

Fig. 4.

Fig. 4

Histopathological analysis of lungs from sgp130Fc-treated SARS-CoV-2 infected mice. Representative images of lung sections from mice of each experimental group. a) H&E stain from dead/euthanized SARS-CoV-2-infected animals (<15 dpi), untreated (vehicle) and sgp130Fc treated. Pictures show multifocal lesions, alveolar haemorrhage (asterisk), oedema (black arrowhead), inflammatory infiltrates (black arrow) (50 X, 200 X and 400 X). b) H&E stain from surviving SARS-CoV-2-infected animals (15 dpi), untreated and sgp130Fc-treated. Inflammatory foci (dash lines) (50 X) and cells, mainly lymphocytes (black arrow) (200 X and 400 X) are indicated. c) Masson's trichrome stain from surviving SARS-CoV-2-infected animals (15 dpi), untreated and sgp130Fc-treated (50 X). Deposits of connective tissue interposed between lymphocytes (black arrow) (200 X and 400 X). d) Number of inflammatory foci per mouse and % of inflammatory foci area per lung section area quantified by ImageJ/FIJI software in surviving mice (15 dpi). Graphs show mean and 95% CI. Statistics were calculated by Student's t-test. p values are indicated in the graphs. e) H&E stain from uninfected animals (mock; 50 X, 200 X and 400 X). Scale bars: 50 X (3000 μm), 200 X (100 μm) and 400 X (50 μm). SARS-CoV-2 + vehicle 15 dpi (n = 8); SARS-CoV-2 + sgp130Fc 15 dpi (n = 12). SARS-CoV-2 + vehicle <15 dpi (n = 9); SARS-CoV-2 + sgp130Fc < 15 dpi (n = 6). Data are combined from two independent experiments. H&E: Haematoxylin Eosin. dpi: days post infection. < 15 dpi: dead/euthanized mice before the end of the experiment. 15 dpi: surviving mice at the end of experiment.