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. 2024 Apr 26;103:105132. doi: 10.1016/j.ebiom.2024.105132

Fig. 6.

Fig. 6

Impact of IL-6 trans-signalling inhibition by sgp130Fc on endothelial cells activation in lungs from SARS-CoV-2 infected surviving mice (15 dpi). Representative immunostaining images of lung sections from surviving groups (15 dpi). a) Immunoreaction of P-Selectin on luminal surface of endothelial cells and platelet aggregates (50 X, 200 X and 400 X). Positive cells were quantified using QuPath software. Data are presented as % of positive cells (mean ± SEM). b) vWF immunostaining in mural thrombus, oedematous fluid in alveoli and endothelial cells. Positive area was quantified using ImageJ/FIJI software. Data are presented as mean ± SEM. c) Immunoreaction of VCAM1 on vascular endothelial cells (50 X, 200 X and 400 X). Positive area was quantified using ImageJ/FIJI software. d) Co-localization of endothelial cells (labelled with isolectin B4) with P-Selectin/vWF/VCAM1 in lung tissues by immunofluorescence (400 X). Scale bars: 50 X (3000 μm), 200 X (100 μm) and 400 X (50 μm). SARS-CoV-2 + vehicle 15 dpi (n = 8); SARS-CoV-2 + sgp130Fc 15 dpi (n = 12). 15 dpi: surviving mice at the end of experiment. All graphs show mean and 95% CI. Statistics were calculated by Student's t-test. p values are indicated in the graphs.