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. 2024 Apr 17;12:1376814. doi: 10.3389/fcell.2024.1376814

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Copyright © 2024 Lv, Wang, Lin, Zheng, Zhang and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Wnt1-Cre;pMes-Fgf18 embryos exhibited severely hypoplastic Meckel’s cartilages. (A–F) Immunostaining was performed on coronal sections of the mandible at E13.5 with antibodies against Sp7 (A,B), Caspase 3 (C,D), and Ki67 (E,F). (G,H) Quantification analysis of the percentage of Caspase 3-positive cells and Ki67-positive cells in the mandible at E13.5. (I) Statistical analysis of the length of Meckel’s cartilage. (J–M) Hematoxylin and eosin (HE) staining of Meckel’s cartilage of embryos at E16.5. (N–Q) The top and side macroscopic views of the lower jaw of mouse embryos at E14.5, following Alcian Blue-Alizarin Red staining. Scale bar = 200 µm (A–F), Scale bar = 400 µm (J–Q), *p < 0.05.