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. 2024 Apr 17;11:1366259. doi: 10.3389/fmolb.2024.1366259

FIGURE 2.

FIGURE 2

IL-1R1 on CD11c+ cells attenuates ischemic AKI. (A) Experimental model: Myel WT and Myel KO mice were subjected to ischemia reperfusion surgery for 6 h or 24 h, followed by measurement of (Created with Biorender.com) (B) Serum creatinine levels and (C) Blood urea nitrogen (BUN) at each indicated timepoints. Dots represent individual samples from each group. Statistical analysis performed by two-way ANOVA with Sidak test. (n = 3 for baseline groups; n = eight to nine mice for 6-h groups; n = 11 for 24-h groups; *p < 0.05, **p < 0.01). (D) Representative 20x periodic acid-Schiff (PAS) stained kidney sections from mice subjected to no I/R (Baseline) or 24 h of I/R (scale bar–50 mm, white arrowhead shows brush border in baseline sections, black arrowhead shows example of dilated tubule in I/R sections) with (E) quantitation of injury scoring of kidney sections by blinded observer (p-value determined by two-way ANOVA). (F) Western blot analysis of Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Myel WT and Myel KO kidneys at baseline and 24-h after I/R. (G) Densitometry analysis of NGAL normalized to internal control (β-actin). Statistical significance between groups was determined by two way ANOVA with Sidak post-test (**p < 0.01).