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. 2024 Apr 17;11:1366259. doi: 10.3389/fmolb.2024.1366259

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Copyright © 2024 Chen, Lu, Whitney, Li, Robson, Blakely, Chi, Crowley and Privratsky.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Myel KO mice show similar numbers of leukocyte cell populations compared to Myel WT mice in the kidney at 24-h after I/R. Mice were subjected to sham or I/R, and kidneys were harvested at 24 h. (A) Experimental workflow for testing leukocytes accumulations by flow cytometry in kidneys of Myel WT and Myel KO mice (Created with Biorender.com). (B) Gating strategy for cell populations. Pseudocolor dot plots for (C) CD45⁺, (D) neutrophils (PMN), (E) Ly6c^hi Monocytes (iMono), (F) CD11b^hi macrophages (CD11b^hi Macs). CD11c⁺ populations include (G) Ly6c^lo Monocytes, (H) conventional dendritic cell (cDC)1, (I) cDC2, (J) F480^hi macrophages. Dots represent individual samples from each group. There were no statistically significant differences between genotypes for any cell population as determined by two-way ANOVA with Sidak test.