TABLE 1.
Ability of different morbilliviruses to downregulate CD46
| Morbillivirus (type of strain) | Strain | % Downregulation (mean ± SD)a |
|---|---|---|
| MV (vaccine) | Human 2/Edmonston | 49 ± 9 |
| RPV (vaccine) | RBOK | 55 ± 4 |
| RPV (laboratory passaged) | Saudi | 67 ± 11 |
| RPV (wild type) | Kabete O | 80 ± 7 |
| Saudi/81 | 66 ± 11 | |
| CDV (vaccine) | Onderstepoort large plaque | 8 ± 6 |
| Onderstepoort small plaque | 6 ± 14 | |
| CDV (wild type) | Belfast dog isolate | 7 ± 19 |
| CDVA75-17 | 5 ± 13 | |
| PPRV (vaccine) | Nigeria 75/1 | 22 ± 6 |
| DMV (wild type) | MUC | 4 ± 8 |
| Ad-H recombinantb | RBOK | 54 ± 9 |
| Ad-N recombinantb | RBOK | 10 ± 14 |
a
Percentage of downregulation of CD46 = 100 − [(MFI of CD46 in infected cells × 100)/(MFI of CD46 in uninfected cells)] where MFI is mean fluorescence intensity. CD46 expression was detected by flow cytometry using a polyclonal antibody. Vero or B95a cells were infected for either 1 or 2 days at an MOI of 1. Results of at least three independent experiments per strain are given.
b
Vero or B95a cells were infected for either 1 or 2 days with the RPV Ad-H or RPV Ad-N recombinant at an MOI of 103.