Fig. 2.

(A) Flow cytometry analysis of the expression levels of CD86 and CD206 in LPS-activated BV2 cells treated with AK123, free FN, AK123/FN or AK123/BSA. (B, C) Mean fluorescence intensity of (B) CD86 and (C) CD206 expression in BV2 cells obtained from (A). (D) Ratio of CD206/CD86 and (E) CLSM images of ROS in LPS-activated BV2 cells after incubated with FN, AK123, AK123/FN or AK123/BSA for 24 h. ELISA analysis of (F) IL-6, (G) IL-1β and (H) TNF-α expression in LPS-activated BV2 cells treated with FN, AK123, AK123/FN or AK123/BSA for 24 h. For A-G, LPS and PBS were used as positive and negative control, respectively, and the [FN] was 20 μg/mL (n = 3 for each group or measurement). In B-D and F-G, ns for no significance, * for p < 0.05, ** for p < 0.01, and *** for p < 0.001, respectively.