Fig. 6.

Cross-sectional area of muscle fibers and neuro-muscular junction analysis of target muscles freezing section. (A) Statistical histograms showed the average cross-sectional area of targeted muscle fibers (n = 3; not less than 9 random fields per rat). (B) Representative images of laminin immunostaining of cross-section of targeted muscles in different groups. (C) Representative images of α-bungarotoxin staining of motor endplates in three groups, showing mature pretzel, intermediate morphology, or immature plaque. Data are presented as Mean ± SEM. ∗, p < 0.05; ∗∗∗, p < 0.001. Scale bar, 50 μm.