Figure 1.

SM truncation is increased in hypoxic endometrial cancer tissues.A, paired tumor (T) and adjacent benign (B) tissue lysates from lean (L) and obese (O) endometrial cancer patients were analyzed by immunoblotting for HIF1α, SM, trunSM (red), and the 14-3-3 housekeeping protein. B and C, graphs depict densitometric quantification of protein levels from (A) normalized to 14-3-3 levels and (C) expressed as a proportion of total SM levels. Data presented as mean ± SD from n = 14 paired tissue sets (∗p ≤ 0.05; ∗∗p ≤ 0.01; two-tailed paired t test versus adjacent benign tissue; normality of distributions confirmed by D’Agostino & Pearson testing (82)). D, Pearson correlation between HIF1α levels in (B) and trunSM levels in (C). Blue line indicates linear regression. HIF1α, hypoxia-inducible factor-1α; SM, squalene monooxygenase; trunSM, truncated SM.