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Diagram of the TK region of the Malawi LiL-20/1 genome, showing the placement and orientation of the TK gene and adjacent genes p10 (DNA binding protein 5-AR) (33), K205R, K145R, and K421R (Ba71V open reading frames) (46); structure of the TK gene deletion transfer vector, p5.3, showing insertion sites into the pBluescript multiple cloning site; and structure of plasmid pLR-TK, containing a 2,164-bp PCR fragment of the intact TK gene and flanking ASFV DNA.
