Table 4.

Effects of different concentrations of arginine on the contents of polyamines and NO in cells and supernatants

Item	Arginine concentrations(mmol/L)									SEM	P value
	0	0.1	0.2	0.4	0.7	1.1	1.6	3.2	6.4		Linear	Quadratic
Intracellular
Spermine (µmol/L)	0.54	1.07	1.20	1.24	1.35	1.37	1.20	1.16	0.84	0.13	0.54	0.04
Spermidine (µmol/L)	0.90	1.37	1.45	1.88	1.95	1.87	1.68	1.65	1.05	0.11	0.79	0.03
Putrescine (µmol/L)	0.43	0.80	0.87	1.00	1.64	1.24	1.01	0.93	0.48	0.08	0.63	0.03
NO (µmol/L)	0.52	0.73	0.83	1.35	1.39	1.43	1.52	1.5	1.46	0.29	0.04	0.13
Cell supernatant
Spermine (µmol/L)	0.001	0.001	0.001	0.002	0.002	0.001	0.001	0.002	0.001	0.0001	0.19	0.89
Spermidine (µmol/L)	0.002	0.001	0.002	0.002	0.002	0.001	0.001	0.003	0.002	0.0001	0.14	0.92
Putrescine (µmol/L)	0.706	0.735	0.698	0.957	0.746	1.226	1.604	1.378	1.940	0.081	0.74	0.07
NO (µmol/L)	0.02	0.04	0.08	0.15	0.19	0.26	0.32	0.28	0.23	0.11	0.09	0.17

SEM, standard error of the mean (n = 4). NO: nitric oxide