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. Author manuscript; available in PMC: 2024 May 1.
Published in final edited form as: Nat Ecol Evol. 2020 Jul 20;4(10):1416–1430. doi: 10.1038/s41559-020-1234-2

Fig. 4 |. Cellular analysis of acute inflammatory response following LPS injection of A. mexicanus HK cells.

Fig. 4 |

a, Experimental set-up of the scRNA-seq experiment using HK from surface fish (Río Choy) and cavefish (Pachón) 3 h postinjection (3 hpi) of either PBS or an LPS mix in the concentrations shown. After processing (for example, removal of mature erythrocytes; see Methods for details), cells were sequenced using 10X Genomics technology (see Methods for details), which resulted in given cell numbers and average genes per cell. b, UMAP projection of PBS- and LPS-injected surface and cavefish. Cell clusters are based on gene enrichment analysis for each cluster (Supplementary Data 5).