FIG. 8.
P58IPK prevents PKR dimerization in the λ repressor fusion system. (A) Coexpression levels of GST fusion proteins. Crude cell extracts from bacteria coexpressing λN-PKR(K296R) and various GST fusions were subjected to SDS-PAGE on a 14% gel and visualized by immunoblot analysis with a polyclonal antibody directed against GST. Positions of molecular size standards are indicated in kilodaltons. (B) Expression of λN-PKR chimeric protein. The panel represents the same blot probed with a polyclonal antibody to PKR. The arrow indicates the position of λN-PKR(K296R). (See also Table 2.)