. Author manuscript; available in PMC: 2025 Mar 1.

Published in final edited form as: Chem Biol Interact. 2024 Feb 15;391:110910. doi: 10.1016/j.cbi.2024.110910

Table 5.

Optimization of the benzimidazole N-alkyl substituent.

				ALDH EC₅₀ (nM) or % Control at 20 µM			CLogP	PEO1 Cell ALDEFLUOR (% inhibition)
No.	R^2,8	R^4,5	R⁶	1A1	1A2	1A3	CLogP	PEO1 Cell ALDEFLUOR (% inhibition)
8	−H,−H			780 ± 78	2200 ± 200	1800 ± 500	4.72	ND
22	−H,−H			620 ± 59	98%	97%	5.18	ND
6	−Cl,−H	−H,−H		190 ± 15	240 ± 57	79 ± 30	4.27	50 ± 4% (10 µM)
23	−Cl,−H	−H,−H		3300 ± 710	4500 ± 990	1500 ± 1200	3.35	ND
24	−Cl,−H	−H,−H		97 ± 29	390 ± 84	250 ± 10	4.17	ND
25	−Cl,−Cl	−H,−H		83 ± 12	45 ± 6	43 ± 2	3.89	97 ± 2% (10 µM) 29 ± 5% (1 µM)