FIGURE 4.

Screening of the Immune-related gene signature. (A) The expression of immune-related DEGs. (B,C) Construction of immune-related gene signature using LASSO and RF. (D) The overlap of genes in two machine learning. (E) Pairs plot showing the relationship between the immune-related gene signature. (F–H) Expression levels of three hub genes in POAG patients compared with healthy controls, *p < 0.05; ***p < 0.001. (I) ROC curve of immune-related hub genes in POAG diagnosis. DEGs, differentially expressed genes; LASSO, least absolute shrinkage and selection operator; RF, random forest; ROC, receiver operating characteristic. *p < 0.05; ***p < 0.001. (J) Nomogram for measuring the significance of immune in POAG based on hub genes. (K) Calibration curve plot for the nomogram. The X-axis represents the predictable probability, and the Y-axis represents the actual probability. Perfect prediction corresponds to the ideal dashed line. The apparent dashed line represents the entire queue, bias-corrected solid line is bias-corrected by bootstrapping (1,000 repetitions) and represents the observed performance of the nomogram. POAG, primary open-angle glaucoma; CON, healthy controls.