Fig 1. RT-QuIC detection of 263K prion seeding activity on stainless steel wires after 1 year.
Stainless-steel wires were coated with 10−3 brain equivalents of 263K or uninfected brain tissue, dried, and inserted in individual wells for RT-QuIC analysis (one wire per well). One set of wires was tested after drying for 24 h (263K, orange; uninfected, black; n = 3); another set (263K only) was tested after 1 y of storage at room temperature (blue; n = 3). ThT fluorescence for each reaction is plotted vs. time.