Fig 3. sfRT-QuIC of stainless steel- or acrylic-bound 263K, CWD, or uninfected brain homogenates.

Panel A-B. sfRT-QuIC of SM collected from a single spot from 263K (red, full line) or uninfected (blue, full line) BH (10⁻⁴ dilution) dried onto stainless steel (A) or acrylic (B) surfaces or in solution (A; red and blue dashed lines). Panel C-D. sfRT-QuIC of a 2 mL SM collection from 8 263K (red) or uninfected (blue) spottings placed in a random pattern within a test circle on stainless steel (C) or acrylic (D). Each collection was tested in quadruplicate. Panel E-F. sfRT-QuIC of SM collected as in C,D from spottings of CWD (red) or uninfected (blue) BH (10⁻⁴ dilutions) from either deer (E) or elk (F). The recombinant PrP substrates used are described in Methods.