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. 2024 May 1;10(18):eadj0604. doi: 10.1126/sciadv.adj0604

Fig. 5. Electrical stimulation regulates macrophage polarization.

Fig. 5.

(A) Schematic diagram of electrical stimulation of the cell. (B) Cytotoxicity of electrical stimulation. (C) Schematic diagram of macrophage polarization. (D) Pro-inflammatory factors under different electrical stimulation times. (E) Anti-inflammatory factors under different electrical stimulation time. (F) Western blot of the expression of key macrophage proteins (CD86, the label of M1 macrophage; CD206, the label of M2 macrophage) and band quantitative analysis. (G) Representative immunofluorescence images of labeled macrophages under different stimulation times. Scale bars, 200 μm. (H) Flow cytometry to detect the polarization of macrophages under different stimulation times. Data are means ± SD (n = 3). **P < 0.01, and ***P < 0.001, ****P < 0.0001. ns, not significant.