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. Author manuscript; available in PMC: 2024 May 1.
Published in final edited form as: Trends Genet. 2023 Mar 25;39(7):560–574. doi: 10.1016/j.tig.2023.02.015

Figure 1. Chemically Inducible Cas9 Cleavage Systems.

Figure 1.

A) CRISPR-Cas9 expression depicted driven by a doxycycline promoter. Cas9 forms a cleavage competent complex with its crRNA and tracrRNA (or sgRNA). B) DD-Cas9: proteosome depicted in multicolor. C) iCas9: cellular localization of iCas9 shown in cyan in response to 4-hydroxytamoxiphen (4-HT). D) ciCas9 depicted with BH3 domain in yellow blocking gRNA loading until the small molecule A3 is introduced for activation. E) FKBP-FRB Cas9: dimerization domains shown in yellow and cyan are independent until the introduction of the small molecule rapamycin which promotes dimerization and activates the complex.