Table 2.

Comparison of Inducible Cas9 Strategies

	Strategy	Stimulator	Induction Speed	Activation Area	Efficiency	Ref.
Chemically Inducible Systems	Dox-Inducible	Doxycycline	Hours to days	N/A	High	2015 Nat Biotech
	FKBP-FRB Dimerization	Rapamycin	Hours	N/A	Moderate	2015 Nat Biotech
	Estrogen Receptor-fused Cas9	4-hydroxytamoxifen	Minutes to hours	N/A	Low	2016 Nat Chem Biol
	Autoinhibitory Cas9	A-385358	Minutes to hours	N/A	Low	2017 Nat Methods
	Degron-fused Cas9	SHIELD1	Hours	N/A	High	2017 Nat Comm
Optically Inducible Systems	Photo-inducible dimerization	Light	Hours	2 mm	Low	2015 Nat Biotech
	Caged Cas9	Light	Hours	5 mm	High	2015 JACS
	Photo-inducible RNA	Light	Seconds to hours	not shown 1 mm 0.2 mm 1 um	High	2016 Angew Chem 2020 Angew Chem 2020 ACS Cent. Sci. 2020 Science