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. 2024 May 1;12:RP91883. doi: 10.7554/eLife.91883

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© 2023, Yang, Chu et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 7. — (A) Diagram showing the schedule of Tamoxifen (TAM) and H89 administration. (B) Immunostaining results of p-Creb in ear sections of the H89-treated mutant and control mice. Scale bars=50 μm. (C) The rescue of the microtia phenotype in the mutant mice by H89. Right panels: Quantitation data. n=3. (D) H/E staining results. Right panels: the thickness of the cartilage and the size of the chondrocytes in the ear of the mutant and control mice. n=3. Right panels: Quantitation data. Scale bars=50 μm. n=3. (E) Immunostaining results for Col1α1 in the ear sections of the H89-treated mutant and control mice. Scale bars=20 μm. Arrows: positive signals. (F) Alkaline phosphatase (ALP) activity was greatly suppressed by H89 in the auricle of the Prrx1^CreERT; Bmpr1a^f/f mice compared to the control mice. Scale bars=50 μm. Arrows: positive signals. G. WB blot also showed that H89 rescued the expression of the osteoblast marker genes Runx2 and osteocalcin in the mutant mice. Right panel: quantitation data. n=3. One-way ANOVA (and nonparametric or mixed) multiple comparisons were applied to evaluate the correlation data in (C, D, and G). p<0.05 was considered as statistically significant.

Figure 7—source data 1. Original file for the western blot in Figure 7G.

elife-91883-fig7-data1.pdf^{(1.1MB, pdf)}

Figure 7—figure supplement 1. — (A) Diagram showing the schedule of Tamoxifen (TAM) and H89 administration. (B) Immunostaining results of p-Creb in ear sections of the H89-treated mutant and control mice. Scale bars=50 μm. (C) The rescue of the microtia phenotype in the mutant mice by H89. Right panels: Quantitation data. n=3. (D) H/E staining results. Right panels: the thickness of the cartilage and the size of the chondrocytes in the ear of the mutant and control mice. n=3. Right panels: Quantitation data. Scale bars=50 μm. n=3. (E) Immunostaining results for Col1α1 in the ear sections of the H89-treated mutant and control mice. Scale bars=20 μm. Arrows: positive signals. (F) Alkaline phosphatase (ALP) activity was greatly suppressed by H89 in the auricle of the Prrx1^CreERT; Bmpr1a^f/f mice compared to the control mice. Scale bars=50 μm. Arrows: positive signals. G. WB blot also showed that H89 rescued the expression of the osteoblast marker genes Runx2 and osteocalcin in the mutant mice. Right panel: quantitation data. n=3. One-way ANOVA (and nonparametric or mixed) multiple comparisons were applied to evaluate the correlation data in (C, D, and G). p<0.05 was considered as statistically significant.

Figure 7—source data 1. Original file for the western blot in Figure 7G.

elife-91883-fig7-data1.pdf^{(1.1MB, pdf)}