FIG. 4.

Wnt-1 induces cytosolic accumulation of β-catenin protein in Rat-1 fibroblasts. Western blot analyses of steady-state β-catenin protein levels in unfractionated (A), cytosolic (B), and membranous (C) protein fractions of wild-type Rat-1 (blank), Rat-1/Wnt-1 (W1), and Rat-1/Wnt-5A (W5A) cells are shown. Cells were uninfected or infected with Ad/Wnt-1 or with the control, Ad/LacZ (MOI = 5). Cell extracts were prepared and fractionated, as described in Materials and Methods. Protein fractions were separated by SDS-PAGE (10% polyacrylamide), transferred onto nitrocellulose, and analyzed by Western blot analysis with anti-β-catenin antibody (Anti-β-Cat).