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. 1998 May;18(5):2474–2485. doi: 10.1128/mcb.18.5.2474

FIG. 5.

FIG. 5

Coexpression of Wnt-5A does not interfere Wnt-1 induction of cytosolic β-catenin in Rat-1 fibroblasts. (A) Expression of Wnt-5A protein in Rat-1 cells at increasing MOIs with Ad/Wnt-5A. Western blot analysis of cell lysates 2 days after infection with Ad/Wnt-5A at an MOI of 5, 10, or 20 or with Ad/Wnt-1 (W1) (MOI = 5) or Ad/LacZ (Z) (MOI = 5) or after mock infection (M) is shown. Wnt-1 and Wnt-5A proteins are detected with anti-HA antibody. (B) Coexpression of Wnt-5A does not interfere with Wnt-1 induction of cytosolic β-catenin in Rat-1 fibroblasts. Western blot analyses of steady-state cytosolic β-catenin protein levels in Rat-1 cells following mock infection (M), infection with Ad/Wnt-1 (W1), Ad/LacZ (Z), or Ad/Wnt-5A (5A), coinfection with Ad/Wnt-1 (MOI = 5) and Ad/Wnt-5A (MOI = 5, 10, or 20), or coinfection with Ad/Wnt-1 (W1) (MOI = 5) and Ad/LacZ (Z) (MOI = 20) are shown. Cell extracts were prepared, fractionated, and analyzed by Western blot analysis with anti-β-catenin antibody (Anti-β-Cat).