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. 2024 Apr 17;20(5):634–645. doi: 10.1038/s41589-024-01580-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, Schematic representation of the RT-QuIC assay. Aggregates derived from the brain tissue of patients suffering with DLB were used to induce αS aggregation. Samples from brains of patients with CBD were used as a negative control. b, Kinetic traces of a 7 µM solution of αS in the presence of CBD seeds (pH 8, 42 °C, shaking at 400 rpm with 1 min intervals, n = 4 replicates; central measure, mean; error, standard deviation (s.d.)). CBD samples were 1% DMSO (blue), 7 µM Anle-138b (teal), parent (orange), I1.01 (purple), I3.02 (red), I3.08 (turquoise) and I4.05 (light blue). Anle-138b, in teal, induces aggregation under this condition. c, Kinetic traces of a 7 µM solution of αS in the presence of DLB seeds (n = 4 replicates; error, s.d.; all other conditions match b). The DLB samples were 1% DMSO (purple), 3.5 µM molecule (blue), 7 µM molecule (teal) and 25 µM molecule (orange). Anle-138b again appears to accelerate rather than inhibit aggregation.