Extended Data Fig. 5. FOXO1 or TCF1 overexpression induces chromatin remodelling in CD19.28ζ and HA.28ζ CAR T cells.

a–e, Bulk ATAC-seq analyses of day 15 tNGFR⁺ CD8⁺ CAR T cells expressing either CD19.28ζ (a–d) or HA.28ζ (e) (n = 3 donors). a, PCA of CD19.28ζ cells. b,c, Rank-ordered plot of differentially accessible TF-binding motifs (P < 0.05 with abs(log₂FC)>0.5) in FOXO1_OE cells (b) and TCF1_OE cells (c) versus tNGFR cells. d,e, Heat maps and hierarchical clustering of mean differential motif accessibility of CD19.28ζ (d) or HA.28ζ (e) cells. Scales show normalized z-scores for each motif. Statistical comparisons were performed using DESeq2 (b–e).