Extended Data Fig. 2. UmbA proteins contain a conserved trypsin-like domain, and construct design and input protein levels for studies of the interactions between proteins in the Umb complex.

a, Alignment of the trypsin-like domain of the UmbA proteins of S. coelicolor. b, Alignment of UmbA1(T) and bovine trypsin. c, Predicted structure and genetic architecture of our construct for the expression of UmbC1(ring). d-f, WB analyses of input samples from IP experiments between the indicated heterologously expressed, tagged (–H, hexahistidine; –V, VSV-G epitope) Umb proteins. Control lanes correspond to beads in the absence of a bait protein. UmbB(Ap) is a UmbB protein from the distantly related species Actinoplanes philippinensis.