FIG. 3.

Western blot analysis of Ace1p levels during Cu ion treatment. Exponential-phase cultures of S. cerevisiae DTY1 were treated with 1, 10, or 100 μM CuSO₄. Five-milliliter samples were taken after 0, 2, 5, 10, 15, 20, 25, 30, 45, 60, 75, and 90 min of Cu treatment. Thirty micrograms of total protein extract was analyzed by immunoblotting with polyclonal antibodies raised against purified recombinant Ace1p from E. coli (rACE1p).