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Effect of increasing Cu ion concentration on CUP1 regulation. Exponential-phase cultures of strain DTY1 were treated with 1 or 5 mM CuSO4. Five-milliliter samples were taken after 0, 15, 30, 45, 60, 90, and 120 min of Cu treatment. RNA was extracted from each sample and analyzed by RNase protection assays. Each reaction contained 20 μg of total RNA. CUP1 and ACT1 mRNAs are indicated by arrows. Quantitation of CUP1 mRNA is shown in the graph.
