FIG. 6.

Nuclear localization of NPc upon fusion of the second ankyrin repeat of IκBα. COS-1 cells were transfected with CMV-based expression vectors encoding NPc (A), NPc–p40-ARD (B), NPc–p40-ARD-114A3 (C), NPc–p40-AR2 (D), NPc–p40-AR2-114A3 (E), or NPc-M9 (F). The NPc fusion proteins contain an N-terminal epitope tag derived from the c-Myc protein. The cellular localization of the NPc proteins in transfected cells was determined by indirect immunofluorescence with anti-myc IgG. The cells shown are representative of more than 200 cells that were positive for the expression of the indicated proteins.