FIG. 8.

Nuclear localization of NPc or PK upon fusion of ARDs. COS-1 cells were transfected with CMV-based expression vectors encoding NPc–MAD3-ARD (A), NPc–53BP2-ARD (B), NPc–GABPβ-ARD (C), NPc–Notch1-ARD (D), PK (E), or PK–53BP2-ARD (F). The NPc and PK fusion proteins contain an N-terminal epitope tag derived from the c-Myc protein. The cellular localization of the NPc and PK fusion proteins in transfected cells was determined by indirect immunofluorescence with anti-myc IgG. The cells shown are representative of more than 200 cells that were positive for the expression of the indicated proteins.