Figure 2.

Fifty-two weeks-old female mice challenged with EtOH has less lipid accumulation in liver than same age male mice. (A) H&E staining in 52-week-old female and male C57BL/6 mice treated with EtOH (6 g/kg) performed in paraffin liver sections. (B) ALT levels measured in serum of 52-week-old female and male mice after EtOH or PBS (U/L) (n = 7–9). (C) ORO staining performed in liver cryosection of 52 weeks-old female and male mice liver treated with EtOH (6 g/kg) or PBS and (D) each quantification. (E) Quantification of triglyceride content in liver (mg TG/mg liver) in liver of 52 weeks-old female and male mice after an EtOH/PBS oral gavage. (F) Serum triglycerides (mg/dL) (n = 5–7). Scale bars: 100 μm. *P < 0.05, **P < 0.01. ns, no statistical difference.