Figure 3.

Fecal microbiota transplantation (FMT) from 52-week-old female donors reduces gut permeability in male mice of the same age after EtOH intoxication. (A) H&E staining of large intestine of 52-week-old female, male and male + FMT mice after EtOH oral gavage. (B) ZO-1 immunofluorescence staining in 52-week-old female, male, and male + FMT mice after EtOH gavage in large intestine cryosections (C) and each quantification. (D) Occludin immunofluorescence staining in large intestine cryosections of 52 weeks-old female, male, and male + FMT mice after EtOH (6 g/kg) oral gavage and (E) each quantification. (n = 4). Scale bars: 100 μm. (F) MUCIN-2 immunofluorescence staining was performed in large intestine of 52 weeks-old female, male, and male + FMT mice after EtOH oral gavage and (G) each quantification (n = 4–7). Scale bars: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001. ns, no statistical difference.