Figure 5.

Fecal microbiota transplantation (FMT) reduces bacterial translocation, infiltration of immune cells and senescence after EtOH challenge in male mice. (A) F4/80 immunofluorescence staining in liver cryosections of 52-week-old female, male, and male + FMT mice after EtOH intoxication and (B) each quantification; and (C) CD11b immunofluorescence staining in liver and (D) each quantification. (E) Tlr4, (F) Tlr2 and (G) Tlr9 mRNA relative expression to Gapdh in liver of 52 weeks-old female, male, and male + FMT after EtOH gavage (H) β-galactosidase immunofluorescent staining performed in liver cryosections of female, male, and male + FMT mice after EtOH oral gavage and (I) each quantification (% of positive cells) (n = 3–4). Scale bars: 100 μm. *P < 0.05, ^**p < 0.01, ^***p < 0.001. ns, no statistical difference.