Figure 6.

Characterization of changes in gut microbiota in FMT-male mice treated with EtOH. (A) Rarefaction curves after 24 h of nanopore-based long-read sequencing of bacterial 16S rRNA in feces from female, male, and male + FMT mice following EtOH treatment. (B) Alpha diversity as measured by Observed-OTUs and Shannon indexes across groups. (C) Hierarchical cluster analysis according to the distribution of phyla within the top 10 more abundant OTUs in each sample. (D) Sankey diagram showing the inferred relationship between groups by relative abundance (%) of top 10 classified OTUs at species level. (E) Proposed model for gender-specific pathophysiology of alcohol intoxication and the influence of female fecal microbiota transplantation (FMT) on the gut-liver axis of male recipient mice. ^*p < 0.05.