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. 2024 Feb 10;161(5):501–511. doi: 10.1093/ajcp/aqad178

FIGURE 1.

FIGURE 1

Identification of extracellular vesicles by flow cytometry. Calibration of a flow cytometer using Megamix-Plus SSC beads to set up gate limits for EV detection. A, Identification of bead subpopulations based on their complexity characteristics and fluorescent intensity. B, The establishment of EV gates at 0.5 µm (upper cloud), 0.24 µm (second cloud), 0.20 µm (third cloud), and 0.16µm (bottom cloud). The defined region for EVs (enclosed within 2 horizontal lines) encompasses the majority of the theoretical large EV size range (0.1-1 µm). C, D, Representative dot plots illustrating the EV subpopulation from a patient diagnosed with venous thromboembolism without cancer. C, Features of EVs carrying TF identified in the bottom right region with a TF marker (CD142+), with analogous isotype-matched negative control antibody (D). EV, extracellular vesicle; FITC, fluorescein isothiocyanate; H, height; IgG1, immunoglobulin G1; SSC, side scatter; TF, tissue factor.