Figure 2.

MP TRAC-CAR T cells produce stem cell memory phenotypes

(A) Manufacturing timeline for TRAC-CAR T cells: MP TRAC-CAR T cells were cultured in TexMACS medium for 3 days during activation and then Immunocult XF medium supplemented with IL-7/IL-15 for 7 days during expansion. Control TRAC-CAR T cells were cultured in only Immunocult XF medium supplemented with IL-2 during activation and expansion. (B) Schematic of the definition of CAR T cell phenotypes: T_SCM (stem cell memory), T_CM (central memory), T_EM (effector memory), T_EFF (effector) as defined by their inherent properties. (C) Bar graphs of the lactate production, glucose consumption, lactate production over glucose consumption, and live T cell fold-change of MP or Control TRAC-CAR T cells during expansion in G-Rex6M plates. Two donors, (lactate/glucose) N_MP = N_Control = 6; (proliferation) N_MP = N_Control = 2. (D) Representative contour plots of CD62L/CCR7 co-expression for CAR⁺/TCR⁻ MP and Control TRAC-CAR T cells for the same conditions. CD62L/CCR7 double-positive cells represent a stem cell memory population (T_Scm) (donor F shown). (E) Bar graph depicting the CD62L/CCR7 double-positive population in CAR⁺ T cells for all conditions. Four donors, N_MP = 4, N_Control = 3. Error bars represent mean and standard deviation. Statistical significance was determined with a paired t test (C), and Welch’s t test (E); ∗p < 0.05, ∗∗∗∗p < 0.0001.