FIGURE 2.

Gasdermin B (GSDMB) promotes RNA mimic or RNA virus-induced interferon-stimulated gene (ISG) induction. a, b) mRNA levels of RANTES and OASL (a) or extracellular RANTES protein levels (b) were measured in BEAS-2B cells with overexpression of GSDMB and transfected with high molecular weight (HMW) polyinosinic-polycytidylic acid (poly(I:C)) for 6 h. c) Expression of RANTES in normal human bronchial epithelial cells (nHBEs) with overexpression of GSDMB after transfection of HMW poly(I:C) for 6 h. d, e) RNA level of RANTES and OASL in BEAS-2B cells with overexpression of GSDMB and transfected with low molecular weight (LMW) poly(I:C) (d) or 5′-triphosphate hairpin RNA (3p-hpRNA) for 6 h (e), respectively. f, g) Expression of RANTES and OASL in BEAS-2B cells with overexpression of GSDMB and infected with respiratory syncytial virus (RSV) line 19 (multiplicity of infection=1, 24 h) (f) or rhinovirus A16 (RV-A16) (multiplicity of infection=1, 72 h) (g). h) Measurements of interferon regulatory factor 3 (IRF3) and TANK binding kinase 1 (TBK1) phosphorylation by immunoblotting in green fluorescent protein (GFP)-overexpressing versus GSDMB-overexpressing cells. i) IRF3 dimerisation in BEAS-2B cells with overexpression of GSDMB after transfection of HMW poly(I:C) for 0, 3 and 6 h. Cells expressing GFP were used as controls. For a–g, mean±sem shown from at least three independent biological replicates. For all immunoblot data, representative results were shown from two independent biological experiments. ns: nonsignificant. **: p<0.01 (two-way ANOVA).